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KMID : 0364219820250020063
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Korean Journal of Zoology
1982 Volume.25 No. 2 p.63 ~ p.70
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Proteolytic Enzyme in the Midgut during Metamorphosis of Pieris rapae L.
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Kim Hak-Ryul
Yoe Sung-Moon
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Abstract
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The activity, properties, and distribution of midgut protease during metamorphosis in Pieris rapae L. are determined using spectrophotometer, ultracentrifuge and agar gel electrophoresis. Proteolytic activity of midgut reaches the peak just before ecdysis in 5th instar and prepupal stages each but 1 day after ecdysis in pupal stage. Also, optimum pH of midgut protease is pH 8.0 in 5th instar stage, pH 6.5 in prepupal stage, and pH 8.5 immediately before emergence respectively. Protease is found mostly in midght tissue in 5th instar stage but thereafter until just before emergence the enzyme only in lumen contents, suggesting that protease is synthesized in midgut tissue during larval stage and then released into lumen during pupation period.
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